A2074
Sigma
Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse
clone RG-16, purified immunoglobulin, lyophilized powder
| Synonym: | Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM) |
Description
| Preparation Note | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Specificity | Binds only rabbit Igs. Does not react with reduced rabbit Igs. |
| Physical form | Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal |
| General description | The antibody reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM which is sensitive to reduction. No cross-reactivity is observed with human IgG, IgA and IgM. |
Properties
| antibody form | purified immunoglobulin |
| clone | RG-16 (monoclonal) |
| form | lyophilized powder |
| does not react with | guinea pig, turkey, rat, goat, canine, feline, bovine, sheep, chicken, horse, human, pig |
| packaging | vial of 0.5 mL |
| application(s) | direct ELISA: 1:30,000 |
| dot blot: 1:10,000 | |
| immunoblotting (chemiluminescent): 1:160,000 | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 | |
| isotype | IgG1 |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 1 |
References
| reference | Herring, A., et al., Reduction of Amyloid Angiopathy and Aβ Plaque Burden after Enriched Housing in TgCRND8 Mice : Involvement of Multiple Pathways Am. J. Pathol. 169, 544-552, (2006) Abstract |
| Henderson, B., et al., Identification of the Exported Proteins of the Oral Opportunistic Pathogen Actinobacillus actinomycetemcomitans by Using Alkaline Phosphatase Fusions Infect. Immun. 69, 2748-2752, (2001) Abstract | |
| Reichard, U., et al., Sedolisins, a New Class of Secreted Proteases from Aspergillus fumigatus with Endoprotease or Tripeptidyl-Peptidase Activity at Acidic pHs Appl. Environ. Microbiol. 72, 1739-1748, (2006) Abstract |
